PrimePCR™ Probe Assay: AES, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050306
Assay Design:   exonic
Chromosome Location:   19:3053711-3053847question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000221561 ENST00000586742 ENST00000327141 ENST00000586839

Gene Information

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho a protein involved in neurogenesis during embryonic development. The encoded protein which belongs to the groucho/TLE family of proteins can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   AES
Gene Name:   amino-terminal enhancer of split
Aliases:   AES-1, AES-2, ESP1, GRG, GRG5, TLE5
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000104964
Entrez:   166
UniGene:   Hs.515053
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download