PrimePCR™ Probe Assay: TLE6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050304
Assay Design:   exonic
Chromosome Location:   19:2978269-2981536question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000246112 ENST00000453329 ENST00000482627 ENST00000452088

Gene Information

Description Not Available

Gene Symbol:   TLE6
Gene Name:   transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila)
Aliases:   FLJ14009, GRG6, MGC14966
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000104953
Entrez:   79816
UniGene:   Hs.334507
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.450000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download