PrimePCR™ Probe Assay: NUCB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NUCB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NUCB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050292
Assay Design:   exonic
Chromosome Location:   19:49404167-49409012question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000304734 ENST00000493763 ENST00000539605 ENST00000538010 ENST00000284116 ENST00000267199 ENST00000324333 ENST00000381971 ENST00000402297 ENST00000397226 ENST00000341761 ENST00000374193 ENST00000313663 ENST00000334126 ENST00000397128 ENST00000405315 ENST00000407032 ENST00000452087 ENST00000411700 ENST00000451312 ENST00000424608 ENST00000263273

Gene Information

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq Jun 2010]

Gene Symbol:   NUCB1
Gene Name:   nucleobindin 1
Aliases:   CALNUC, DKFZp686A15286, FLJ40471, NUC
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000104805
Entrez:   4924
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.580000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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