PrimePCR™ Probe Assay: DCTN6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050282
Assay Design:   exonic
Chromosome Location:   8:30038005-30038130question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000221114 ENST00000520829

Gene Information

The protein encoded by this gene contains an RGD (Arg-Gly-Asp) motif in the N-terminal region which confers adhesive properties to macromolecular proteins like fibronectin. It shares a high degree of sequence similarity with the mouse homolog which has been suggested to play a role in mitochondrial biogenesis. The exact biological function of this gene is not known. [provided by RefSeq Jul 2008]

Gene Symbol:   DCTN6
Gene Name:   dynactin 6
Aliases:   WS-3, WS3, p27
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000104671
Entrez:   10671
UniGene:   Hs.158427
Chromosome Mapping:   8p12-p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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