PrimePCR™ Probe Assay: NBN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050264
Assay Design:   exonic
Chromosome Location:   8:90993024-90993629question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000265433 ENST00000409330 ENST00000517772 ENST00000517337

Gene Information

Mutations in this gene are associated with Nijmegen breakage syndrome an autosomal recessive chromosomal instability syndrome characterized by microcephaly growth retardation immunodeficiency and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq Jul 2008]

Gene Symbol:   NBN
Gene Name:   nibrin
Aliases:   AT-V1, AT-V2, ATV, FLJ10155, MGC87362, NBS, NBS1, P95
RefSeq:   NC_000008.10 NG_008860.1 NT_008046.16
Ensembl:   ENSG00000104320
Entrez:   4683
UniGene:   Hs.492208
Chromosome Mapping:   8q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.690000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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