PrimePCR™ Probe Assay: ACSBG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050244
Assay Design:   exonic
Chromosome Location:   15:78486854-78486980question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000258873 ENST00000559114 ENST00000559241 ENST00000558130

Gene Information

The protein encoded by this gene possesses long-chain acyl-CoA synthetase activity. It is thought to play a central role in brain very long-chain fatty acids metabolism and myelinogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   ACSBG1
Gene Name:   acyl-CoA synthetase bubblegum family member 1
Aliases:   BG, BG1, BGM, FLJ30320, GR-LACS, KIAA0631, LPD, MGC14352
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000103740
Entrez:   23205
UniGene:   Hs.655760
Chromosome Mapping:   15q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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