PrimePCR™ Probe Assay: BCKDK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BCKDK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BCKDK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050242
Assay Design:   exonic
Chromosome Location:   16:31120426-31120520question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000347755 ENST00000411829 ENST00000340313 ENST00000394217 ENST00000568392 ENST00000570268 ENST00000565865 ENST00000419454 ENST00000435347 ENST00000561234 ENST00000416165 ENST00000560846 ENST00000324417 ENST00000455687 ENST00000242159 ENST00000448415 ENST00000436026 ENST00000528838 ENST00000533240 ENST00000400780 ENST00000564088 ENST00000382450 ENST00000569417 ENST00000325811 ENST00000297991 ENST00000394951 ENST00000394950 ENST00000287507 ENST00000219794 ENST00000561755 ENST00000567676 ENST

Gene Information

Description Not Available

Gene Symbol:   BCKDK
Gene Name:   branched chain ketoacid dehydrogenase kinase
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103507
Entrez:   10295
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 39.780000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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