PrimePCR™ Probe Assay: TRADD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050206
Assay Design:   exonic
Chromosome Location:   16:67188148-67188282question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000345057 ENST00000486556

Gene Information

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2 reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2 and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1 and is involved in the Fas-induced cell death pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   TRADD
Gene Name:   TNFRSF1A-associated via death domain
Aliases:   Hs.89862, MGC11078
RefSeq:   NC_000016.9 NG_009294.1 NT_010498.15
Ensembl:   ENSG00000102871
Entrez:   8717
UniGene:   Hs.460996
Chromosome Mapping:   16q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.030000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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