PrimePCR™ Probe Assay: BEX4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050187
Assay Design:   exonic
Chromosome Location:   X:102471668-102471806question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000372695 ENST00000372691

Gene Information

This gene is a member of the brain expressed X-linked gene family. The proteins encoded by some of the other members of this family act as transcription elongation factors which allow RNA polymerase II to escape pausing during elongation. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Aug 2011]

Gene Symbol:   BEX4
Gene Name:   brain expressed, X-linked 4
Aliases:   BEXL1, FLJ10097
RefSeq:   NC_000023.10 NG_021339.1 NT_011651.17
Ensembl:   ENSG00000102409
Entrez:   56271
UniGene:   Hs.184736
Chromosome Mapping:   Xq22.1-q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.050000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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