PrimePCR™ Probe Assay: GABRE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050182
Assay Design:   exonic
Chromosome Location:   X:151131009-151138149question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000370328 ENST00000370325 ENST00000393914

Gene Information

The product of this gene belongs to the ligand-gated ionic channel (TC 1.A.9) family. It encodes the gamma-aminobutyric acid (GABA) A receptor which is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes an epsilon subunit. It is mapped to chromosome Xq28 in a cluster comprised of genes encoding alpha 3 beta 4 and theta subunits of the same receptor. Alternatively spliced transcript variants have been identified but only one is thought to encode a protein. [provided by RefSeq Oct 2008]

Gene Symbol:   GABRE
Gene Name:   gamma-aminobutyric acid (GABA) A receptor, epsilon
Aliases:   Not Available
RefSeq:   NC_000023.10 NG_012511.1 NT_167198.1
Ensembl:   ENSG00000102287
Entrez:   2564
UniGene:   Hs.22785
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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