PrimePCR™ Probe Assay: RAE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050105
Assay Design:   exonic
Chromosome Location:   20:55929772-55929868question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000395841 ENST00000371242 ENST00000527947 ENST00000411894 ENST00000429339 ENST00000395840 ENST00000452119

Gene Information

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs and has been shown to localize to distinct foci in the nucleoplasm to the nuclear rim and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RAE1
Gene Name:   RAE1 RNA export 1 homolog (S. pombe)
Aliases:   FLJ30608, MGC117333, MGC126076, MGC126077, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101146
Entrez:   8480
UniGene:   Hs.371698
Chromosome Mapping:   20q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download