PrimePCR™ Probe Assay: CSTF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050104
Assay Design:   exonic
Chromosome Location:   20:54972395-54972538question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000415828 ENST00000217109 ENST00000428552 ENST00000452950

Gene Information

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits this protein contains transducin-like repeats. Several transcript variants with different 5' UTR but encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CSTF1
Gene Name:   cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Aliases:   CstF-50, CstFp50
RefSeq:   NC_000020.10 NG_012133.1 NT_011362.10
Ensembl:   ENSG00000101138
Entrez:   1477
UniGene:   Hs.172865
Chromosome Mapping:   20q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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