PrimePCR™ Probe Assay: ZDHHC8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049986
Assay Design:   exonic
Chromosome Location:   22:20128436-20128525question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000334554 ENST00000320602 ENST00000405930

Gene Information

This gene encodes a four transmembrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein may function as a palmitoyltransferase. Defects in this gene may be associated with a susceptibility to schizophrenia. Alternate splicing of this gene results in multiple transcript variants. A pseudogene of this gene is found on chromosome 22.[provided by RefSeq May 2010]

Gene Symbol:   ZDHHC8
Gene Name:   zinc finger, DHHC-type containing 8
Aliases:   DHHC8, ZDHHCL1, ZNF378
RefSeq:   NC_000022.10 NG_021420.1 NT_011519.10
Ensembl:   ENSG00000099904
Entrez:   29801
UniGene:   Hs.63128
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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