PrimePCR™ Probe Assay: PDE6C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049946
Assay Design:   exonic
Chromosome Location:   10:95372364-95372460question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000371447

Gene Information

This gene encodes the alpha-prime subunit of cone phosphodiesterase which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11 13 and 15 kDa. Mutations in this gene are associated with cone dystrophy type 4 (COD4). [provided by RefSeq Mar 2010]

Gene Symbol:   PDE6C
Gene Name:   phosphodiesterase 6C, cGMP-specific, cone, alpha prime
Aliases:   COD4, PDEA2
RefSeq:   NC_000010.10 NG_016752.1 NT_030059.13
Ensembl:   ENSG00000095464
Entrez:   5146
UniGene:   Hs.658121
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download