PrimePCR™ Probe Assay: MYO15A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYO15A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYO15A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049898
Assay Design:   exonic
Chromosome Location:   17:18064651-18064741question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000598585 ENST00000376918 ENST00000334976 ENST00000377680 ENST00000324501 ENST00000205890 ENST00000418233 ENST00000556535 ENST00000578472 ENST00000579848 ENST00000451725 ENST00000596508 ENST00000368457 ENST00000537500 ENST00000594210 ENST00000596945

Gene Information

This gene encodes an unconventional myosin. This protein differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in this gene have been associated with profound congenital neurosensory nonsyndromal deafness. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and this gene have been identified but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described but their full length sequences have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   MYO15A
Gene Name:   myosin XVA
Aliases:   DFNB3, DKFZp686N18198, FLJ17274, FLJ31311, MYO15
RefSeq:   NC_000017.10 NG_011634.1 NT_010718.16
Ensembl:   ENSG00000091536
Entrez:   51168
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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