PrimePCR™ Probe Assay: CEACAM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CEACAM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CEACAM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049700
Assay Design:   exonic
Chromosome Location:   19:43016508-43016597question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000352591 ENST00000161559 ENST00000403444 ENST00000403461 ENST00000358394 ENST00000599389 ENST00000351134 ENST00000308072 ENST00000291532 ENST00000380399 ENST00000361445

Gene Information

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family the CEA cell adhesion molecules and the pregnancy-specific glycoproteins are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently it was found to be a cell-cell adhesion molecule detected on leukocytes epithelia and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein including roles in the differentiation and arrangement of tissue three-dimensional structure angiogenesis apoptosis tumor suppression metastasis and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported but the full-length nature of all variants has not been defined. [provided by RefSeq May 2010]

Gene Symbol:   CEACAM1
Gene Name:   carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Aliases:   BGP, BGP1, BGPI
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000079385
Entrez:   634
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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