PrimePCR™ Probe Assay: RBM7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049641
Assay Design:   exonic
Chromosome Location:   11:114278486-114278635question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000540163 ENST00000375490 ENST00000541475 ENST00000545678

Gene Information

Description Not Available

Gene Symbol:   RBM7
Gene Name:   RNA binding motif protein 7
Aliases:   FLJ11153
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000076053
Entrez:   10179
UniGene:   Hs.7527
Chromosome Mapping:   11q23.1-q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.390000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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