PrimePCR™ Probe Assay: SNCB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SNCB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNCB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049611
Assay Design:   exonic
Chromosome Location:   5:176047838-176053410question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000393340 ENST00000427865 ENST00000446023 ENST00000307826 ENST00000333055 ENST00000406427 ENST00000434090 ENST00000277531 ENST00000250896 ENST00000327141 ENST00000369886 ENST00000391984 ENST00000283351 ENST00000582539 ENST00000262623 ENST00000267406 ENST00000310112 ENST00000393693 ENST00000510387 ENST00000506696

Gene Information

The protein encoded by this gene is highly homologous to alpha-synuclein. These proteins are abundantly expressed in the brain and putatively inhibit phospholipase D2 selectively. The encoded protein which may play a role in neuronal plasticity is abundant in neurofibrillary lesions of patients with Alzheimer disease. This protein has been shown to be highly expressed in the substantia nigra of the brain a region of neuronal degeneration in patients with Parkinson disease; however no direct relation to Parkinson disease has been established. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SNCB
Gene Name:   synuclein, beta
Aliases:   Not Available
RefSeq:   NC_000005.9 NG_012131.1 NT_023133.13
Ensembl:   ENSG00000074317
Entrez:   6620
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.690000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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