PrimePCR™ Probe Assay: CYP2W1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049593
Assay Design:   exonic
Chromosome Location:   7:1027927-1028037question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000296662 ENST00000308919 ENST00000340150 ENST00000415893

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2W1
Gene Name:   cytochrome P450, family 2, subfamily W, polypeptide 1
Aliases:   MGC34287
RefSeq:   NC_000007.13 NG_007934.1 NT_007819.17
Ensembl:   ENSG00000073067
Entrez:   54905
UniGene:   Hs.272795
Chromosome Mapping:   7p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download