PrimePCR™ Probe Assay: TRPM5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TRPM5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRPM5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049566
Assay Design:   exonic
Chromosome Location:   11:2439398-2439493question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000533881 ENST00000155858 ENST00000533060 ENST00000528453 ENST00000452833

Gene Information

This gene encodes a member of the transient receptor potential (TRP) protein family which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction and has characteristics of a calcium-activated non-selective cation channel that carries Na+ K+ and Cs+ ions equally well but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+) and inhibited by higher concentrations. It is also a highly temperature-sensitive heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5 and has been shown to be imprinted with exclusive expression from the paternal allele. [provided by RefSeq Oct 2010]

Gene Symbol:   TRPM5
Gene Name:   transient receptor potential cation channel, subfamily M, member 5
Aliases:   LTRPC5, MTR1
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000070985
Entrez:   29850
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.810000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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