PrimePCR™ Probe Assay: ATP11A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP11A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP11A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049535
Assay Design:   exonic
Chromosome Location:   13:113530133-113530222question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000540372 ENST00000250699 ENST00000582330 ENST00000335464 ENST00000370018 ENST00000508582 ENST00000360203 ENST00000318100 ENST00000261978 ENST00000556690 ENST00000315970 ENST00000458659 ENST00000501704 ENST00000487903 ENST00000419631 ENST00000415301 ENST00000375630 ENST00000375645 ENST00000283558 ENST00000264405 ENST00000542454 ENST00000554311 ENST00000539359 ENST00000306005 ENST00000295897 ENST00000415165 ENST00000503124 ENST00000509063 ENST00000401494 ENST00000511370 ENST00000358024 ENST

Gene Information

The protein encoded by this gene is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP11A
Gene Name:   ATPase, class VI, type 11A
Aliases:   ATPIH, ATPIS
RefSeq:   NC_000013.10 NT_027140.6
Ensembl:   ENSG00000068650
Entrez:   23250
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 37.200000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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