PrimePCR™ Probe Assay: IDH3G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IDH3G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IDH3G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049526
Assay Design:   exonic
Chromosome Location:   X:153052530-153052945question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000379144 ENST00000337573 ENST00000370092 ENST00000217901 ENST00000454076 ENST00000424541 ENST00000370093 ENST00000427365 ENST00000444450 ENST00000444338 ENST00000600403 ENST00000593996 ENST00000596840 ENST00000600205 ENST00000598838 ENST00000596495 ENST00000598003 ENST00000596505

Gene Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits one beta subunit and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described but only some of their full length natures have been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   IDH3G
Gene Name:   isocitrate dehydrogenase 3 (NAD+) gamma
Aliases:   H-IDHG
RefSeq:   NC_000023.10 NG_016329.1 NT_167198.1
Ensembl:   ENSG00000067829
Entrez:   3421
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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