PrimePCR™ Probe Assay: GSTO2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049489
Assay Design:   exonic
Chromosome Location:   10:106037675-106037800question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000338595 ENST00000369707 ENST00000450629 ENST00000401888 ENST00000429569

Gene Information

The protein encoded by this gene is an omega class glutathione S-transferase (GST). GSTs are involved in the metabolism of xenobiotics and carcinogens. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jul 2010]

Gene Symbol:   GSTO2
Gene Name:   glutathione S-transferase omega 2
Aliases:   bA127L20.1
RefSeq:   NC_000010.10 NG_023362.1 NT_030059.13 NG_023363.1
Ensembl:   ENSG00000065621
Entrez:   119391
UniGene:   Hs.203634
Chromosome Mapping:   10q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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