PrimePCR™ Probe Assay: HMG20B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HMG20B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HMG20B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049477
Assay Design:   exonic
Chromosome Location:   19:3574391-3574565question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000395290 ENST00000392411 ENST00000416299 ENST00000321300 ENST00000435091 ENST00000575245 ENST00000575712 ENST00000428708 ENST00000321280 ENST00000379295 ENST00000533881 ENST00000155858 ENST00000533060 ENST00000528453 ENST00000452833 ENST00000333762 ENST00000300527 ENST00000416526 ENST00000333651 ENST00000417382 ENST00000453933 ENST00000262949 ENST00000286744 ENST00000567476 ENST00000572705 ENST00000399759 ENST00000571088 ENST00000399756 ENST00000425167 ENST00000576351 ENST00000310522 ENST

Gene Information

Description Not Available

Gene Symbol:   HMG20B
Gene Name:   high mobility group 20B
Aliases:   BRAF25, BRAF35, FLJ26127, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000064961
Entrez:   10362
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 38.710000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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