PrimePCR™ Probe Assay: GPM6B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049362
Assay Design:   exonic
Chromosome Location:   X:13803749-13803922question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000316715 ENST00000454189 ENST00000493677 ENST00000355135 ENST00000356942 ENST00000398361 ENST00000493085 ENST00000468080 ENST00000475307

Gene Information

This gene encodes a membrane glycoprotein that belongs to the proteolipid protein family. Proteolipid protein family members are expressed in most brain regions and are thought to be involved in cellular housekeeping functions such as membrane trafficking and cell-to-cell communication. [provided by RefSeq Apr 2009]

Gene Symbol:   GPM6B
Gene Name:   glycoprotein M6B
Aliases:   M6B, MGC17150, MGC54284
RefSeq:   NC_000023.10 NG_011988.1 NT_167197.1 NG_008872.1
Ensembl:   ENSG00000046653
Entrez:   2824
UniGene:   Hs.495710
Chromosome Mapping:   Xp22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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