PrimePCR™ Probe Assay: TRIT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049358
Assay Design:   exonic
Chromosome Location:   1:40307476-40309773question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000441669 ENST00000316891 ENST00000372818 ENST00000046894 ENST00000372825 ENST00000545233 ENST00000537440 ENST00000537223 ENST00000541099

Gene Information

Description Not Available

Gene Symbol:   TRIT1
Gene Name:   tRNA isopentenyltransferase 1
Aliases:   FLJ20061, IPT, MGC149242, MGC149243, MOD5
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000043514
Entrez:   54802
UniGene:   Hs.356554
Chromosome Mapping:   1p34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.380000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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