PrimePCR™ Probe Assay: CLEC16A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLEC16A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLEC16A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049337
Assay Design:   exonic
Chromosome Location:   16:11219846-11219939question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000451127 ENST00000341184 ENST00000301904 ENST00000444486 ENST00000602424 ENST00000162023 ENST00000403084 ENST00000409224 ENST00000424583 ENST00000410050 ENST00000371706 ENST00000313050 ENST00000290037 ENST00000431893 ENST00000320389 ENST00000498729 ENST00000505785 ENST00000252744 ENST00000218436 ENST00000372589 ENST00000372585 ENST00000372574 ENST00000436107 ENST00000409790 ENST00000409552 ENST00000428742 ENST00000436973 ENST00000261657 ENST00000540649 ENST00000445369 ENST00000320634 ENST

Gene Information

This gene encodes a member of the C-type lectin domain containing family. Single nucleotide polymorphisms in introns of this gene have been associated with diabetes mellitus multiple sclerosis and rheumatoid arthritis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   CLEC16A
Gene Name:   C-type lectin domain family 16, member A
Aliases:   Gop-1, KIAA0350, MGC111457
RefSeq:   NC_000016.9 NG_016757.1 NT_010393.16
Ensembl:   ENSG00000038532
Entrez:   23274
Chromosome Mapping:   16p13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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