PrimePCR™ Probe Assay: BTN3A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BTN3A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BTN3A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049298
Assay Design:   exonic
Chromosome Location:   6:26406236-26406329question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000233336 ENST00000532865 ENST00000530653 ENST00000527417 ENST00000527422 ENST00000356386 ENST00000377708 ENST00000396948 ENST00000396934 ENST00000508906 ENST00000476549 ENST00000289361 ENST00000450085 ENST00000425234 ENST00000506698 ENST00000414912 ENST00000494393 ENST00000482451 ENST00000244519 ENST00000471353 ENST00000361232 ENST00000487627 ENST00000496719 ENST00000490254 ENST00000487272 ENST00000339789

Gene Information

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g. BTN2A1; MIM 613590) and BTN3 (e.g. BNT3A1) genes which have undergone tandem duplication resulting in 3 copies of each (summary by Smith et al. 2010 [PubMed 20208008]).[supplied by OMIM Nov 2010]

Gene Symbol:   BTN3A1
Gene Name:   butyrophilin, subfamily 3, member A1
Aliases:   BT3.1, BTF5, CD277, MGC141880
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000026950
Entrez:   11119
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.210000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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