PrimePCR™ Probe Assay: RALBP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RALBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RALBP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049270
Assay Design:   exonic
Chromosome Location:   18:9475148-9513055question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000019317 ENST00000585015 ENST00000383432 ENST00000458039 ENST00000577221

Gene Information

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins such as RAL have GDP-bound inactive and GTP-bound active forms which shift from the inactive to the active state through the action of RALGDS (MIM 601619) which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig 2003 [PubMed 12888294]).[supplied by OMIM Nov 2010]

Gene Symbol:   RALBP1
Gene Name:   ralA binding protein 1
Aliases:   RIP1, RLIP1, RLIP76
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000017797
Entrez:   10928
Chromosome Mapping:   18p11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.360000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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