PrimePCR™ Probe Assay: SRCIN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SRCIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRCIN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049267
Assay Design:   exonic
Chromosome Location:   17:36707515-36707604question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000392619 ENST00000359323 ENST00000404898 ENST00000402142 ENST00000336649 ENST00000400164 ENST00000586406 ENST00000565365 ENST00000562477 ENST00000394048 ENST00000267113 ENST00000541590 ENST00000546954 ENST00000278903 ENST00000343678 ENST00000264659 ENST00000578925 ENST00000542707 ENST00000296452 ENST00000221347 ENST00000316005 ENST00000358965 ENST00000393695 ENST00000361256 ENST00000401886 ENST00000398042 ENST00000327374 ENST00000401833 ENST00000432883 ENST00000456048 ENST00000398633 ENST

Gene Information

Description Not Available

Gene Symbol:   SRCIN1
Gene Name:   SRC kinase signaling inhibitor 1
Aliases:   KIAA1684, P140, SNIP
RefSeq:   NC_000017.10 NT_010783.15 NW_003315948.1
Ensembl:   ENSG00000017373
Entrez:   80725
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.440000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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