PrimePCR™ Probe Assay: MAMLD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049255
Assay Design:   exonic
Chromosome Location:   X:149678298-149680356question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000370401 ENST00000432680 ENST00000262858 ENST00000426613 ENST00000455522 ENST00000596168 ENST00000599483 ENST00000599586 ENST00000600812 ENST00000593521

Gene Information

This gene encodes a mastermind-like domain containing protein. This protein may function as a transcriptional co-activator. Mutations in this gene are the cause of X-linked hypospadias type 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq Apr 2010]

Gene Symbol:   MAMLD1
Gene Name:   mastermind-like domain containing 1
Aliases:   CG1, CXorf6, F18, HYSP2
RefSeq:   NC_000023.10 NT_167198.1 NG_017093.1
Ensembl:   ENSG00000013619
Entrez:   10046
UniGene:   Hs.20136
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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