PrimePCR™ Probe Assay: NFIX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NFIX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NFIX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049212
Assay Design:   exonic
Chromosome Location:   19:13136107-13136310question
Amplicon Length:   174
Splice Variants Targeted:   ENST00000413454 ENST00000352066 ENST00000422090 ENST00000397661 ENST00000592199 ENST00000590027 ENST00000587760 ENST00000585575 ENST00000360105 ENST00000588228 ENST00000591028 ENST00000586873 ENST00000587260 ENST00000358552 ENST00000279249 ENST00000544348 ENST00000371191 ENST00000403491 ENST00000485903 ENST00000371185 ENST00000371184 ENST00000371187 ENST00000407417 ENST00000371189 ENST00000589123 ENST00000395111 ENST00000586919 ENST00000341919 ENST00000590282 ENST00000443272 ENST00000346156 ENST

Gene Information

Description Not Available

Gene Symbol:   NFIX
Gene Name:   nuclear factor I/X (CCAAT-binding transcription factor)
Aliases:   NF1A
RefSeq:   NC_000019.9 NG_027756.1 NT_011295.11
Ensembl:   ENSG00000008441
Entrez:   4784
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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