PrimePCR™ Probe Assay: TNFRSF12A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049182
Assay Design:   exonic
Chromosome Location:   16:3071966-3072089question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000573001 ENST00000326577 ENST00000341627 ENST00000575124

Gene Information

Description Not Available

Gene Symbol:   TNFRSF12A
Gene Name:   tumor necrosis factor receptor superfamily, member 12A
Aliases:   CD266, FN14, TWEAKR
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000006327
Entrez:   51330
UniGene:   Hs.355899
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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