PrimePCR™ Probe Assay: POLR2J, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049159
Assay Design:   exonic
Chromosome Location:   7:102113591-102113729question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000292614

Gene Information

This gene encodes a subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2J
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
Aliases:   MGC71910, POLR2J1, RPB11, RPB11A, RPB11m, hRPB14
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000005075
Entrez:   5439
UniGene:   Hs.654952
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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