PrimePCR™ Probe Assay: CYP26B1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP26B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP26B1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049141
Assay Design:   exonic
Chromosome Location:   2:72362292-72362387question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000361125 ENST00000361368 ENST00000572681 ENST00000586783 ENST00000346736 ENST00000589393 ENST00000454313 ENST00000352035 ENST00000590151 ENST00000393896 ENST00000353196 ENST00000570896 ENST00000576812 ENST00000311668 ENST00000322340 ENST00000237612 ENST00000382751 ENST00000001146 ENST00000412253 ENST00000474509 ENST00000546307 ENST00000407683 ENST00000405370 ENST00000404257 ENST00000391969 ENST00000325935 ENST00000598234 ENST00000344649 ENST00000338733 ENST00000413695 ENST00000430560 ENST

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and the synthesis of cholesterol steroids and other lipids. The enzyme encoded by this gene is involved in the specific inactivation of all-trans-retinoic acid to hydroxylated forms such as 4-oxo- 4-OH- and 18-OH-all-trans-retinoic acid. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP26B1
Gene Name:   cytochrome P450, family 26, subfamily B, polypeptide 1
Aliases:   CYP26A2, DKFZp686G0638, MGC129613, P450RAI-2
RefSeq:   NC_000002.11 NG_007957.1 NT_022184.15
Ensembl:   ENSG00000003137
Entrez:   56603
Chromosome Mapping:   2p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 36.590000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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