PrimePCR™ Probe Assay: LASP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049138
Assay Design:   exonic
Chromosome Location:   17:37046736-37054764question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000318008 ENST00000419929 ENST00000435347 ENST00000433206

Gene Information

This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization. [provided by RefSeq Jan 2009]

Gene Symbol:   LASP1
Gene Name:   LIM and SH3 protein 1
Aliases:   Lasp-1, MLN50
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000002834
Entrez:   3927
UniGene:   Hs.548018
Chromosome Mapping:   17q11-q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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