PrimePCR™ Probe Assay: CT47A4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CT47A4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CT47A4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041858
Assay Design:   exonic
Chromosome Location:   X:120104833-120104990question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000382622 ENST00000452611 ENST00000256339 ENST00000555664 ENST00000553484 ENST00000393151 ENST00000221347 ENST00000325888 ENST00000346177 ENST00000371084 ENST00000205890 ENST00000255008 ENST00000371311 ENST00000419982 ENST00000416816 ENST00000457020 ENST00000430448 ENST00000417256 ENST00000457977 ENST00000434883 ENST00000439466 ENST00000419194 ENST00000443600 ENST00000415858 ENST00000441330 ENST00000433030 ENST00000434414 ENST00000371280 ENST00000458218 ENST00000360461 ENST00000565899

Gene Information

This locus represents a member of the cancer/testis gene family 47. This family also known as CT47 is comprised of 13 nearly identical loci clustered at Xq24. [provided by RefSeq Sep 2010]

Gene Symbol:   CT47A4
Gene Name:   cancer/testis antigen family 47, member A4
Aliases:   CT47.4, CT47A1, CT47A10, CT47A11, CT47A12, CT47A2, CT47A3, CT47A5, CT47A6, CT47A7, CT47A8, CT47A9
RefSeq:   NC_000023.10 NG_027740.1 NG_027741.1 NT_011786.16
Ensembl:   ENSG00000230594
Entrez:   728075
Chromosome Mapping:   Xq24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 32.960000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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