PrimePCR™ Probe Assay: RP11-7I15.5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RP11-7I15.5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-7I15.5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041845
Assay Design:   exonic
Chromosome Location:   11:77790769-77790858question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000528251 ENST00000530054 ENST00000281031 ENST00000527806 ENST00000534029 ENST00000525085 ENST00000528164 ENST00000558014 ENST00000316364 ENST00000354744 ENST00000358066 ENST00000389428 ENST00000537942 ENST00000389433 ENST00000389432 ENST00000355997

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring NDUFC2 (NADH dehydrogenase (ubiquinone) 1 subcomplex unknown 2 14.5kDa) and KCTD14 (potassium channel tetramerisation domain containing 14) genes on chromosome 11. The read-through transcripts share sequence identity with the upstream gene product and one variant has a frameshifted C-terminal region derived from the downstream gene exons. [provided by RefSeq Feb 2011]

Gene Symbol:   RP11-7I15.5
Gene Name:   NDUFC2-KCTD14 protein isoform 2
Aliases:   FLJ94527
RefSeq:   Not Available
Ensembl:   ENSG00000259112
Entrez:   100532726
Chromosome Mapping:   11q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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