PrimePCR™ Probe Assay: PNMA6D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PNMA6D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PNMA6D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041816
Assay Design:   exonic
Chromosome Location:   X:152244319-152244467question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000309117 ENST00000288304 ENST00000404891 ENST00000472232 ENST00000525144 ENST00000529097 ENST00000525704 ENST00000468418 ENST00000361582 ENST00000463306 ENST00000306117 ENST00000537075 ENST00000252997 ENST00000176763 ENST00000261799 ENST00000560540 ENST00000290894 ENST00000560734 ENST00000560413 ENST00000458022 ENST00000318390 ENST00000229335 ENST00000543081 ENST00000405995 ENST00000320547 ENST00000357304 ENST00000392108 ENST00000421798 ENST00000453825 ENST00000535416 ENST00000538162 ENST

Gene Information

Description Not Available

Gene Symbol:   PNMA6D
Gene Name:   paraneoplastic antigen like 6D
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000257088
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.790000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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