PrimePCR™ Probe Assay: HIST1H4J, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HIST1H4J, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIST1H4J, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041697
Assay Design:   exonic
Chromosome Location:   6:27792014-27792114question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000497164 ENST00000360134 ENST00000485287 ENST00000335504 ENST00000431659 ENST00000375626 ENST00000416960 ENST00000377803 ENST00000360441 ENST00000377745 ENST00000342505 ENST00000359128 ENST00000295874 ENST00000474889 ENST00000296849 ENST00000274150 ENST00000537972 ENST00000277541 ENST00000256935 ENST00000520908 ENST00000540750 ENST00000354348 ENST00000585201 ENST00000355057 ENST00000357549 ENST00000355981 ENST00000056217 ENST00000474817 ENST00000471847 ENST00000216181 ENST00000369165 ENST

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H4J
Gene Name:   histone cluster 1, H4j
Aliases:   H4/e, H4F2iv, H4FE, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4, MGC166960, MGC29783, dJ160A22.2
RefSeq:   NC_000006.11 NG_000009.2 NT_007592.15
Ensembl:   ENSG00000197238
Entrez:   8363
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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