PrimePCR™ Probe Assay: NDUFA4L2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NDUFA4L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDUFA4L2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041686
Assay Design:   exonic
Chromosome Location:   12:57628715-57628861question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000579545 ENST00000576850 ENST00000577316 ENST00000578158 ENST00000226225 ENST00000582302 ENST00000550422 ENST00000357869 ENST00000554503 ENST00000393825 ENST00000436083 ENST00000356987 ENST00000409858 ENST00000360271 ENST00000321617

Gene Information

Description Not Available

Gene Symbol:   NDUFA4L2
Gene Name:   NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Aliases:   FLJ26118, NUOMS
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000185633
Entrez:   56901
Chromosome Mapping:   12q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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