PrimePCR™ Probe Assay: TUBA1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TUBA1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TUBA1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041666
Assay Design:   exonic
Chromosome Location:   12:49580099-49580217question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000304734 ENST00000493763 ENST00000511831 ENST00000576436 ENST00000569083 ENST00000455915 ENST00000594154 ENST00000316027 ENST00000330423 ENST00000416740 ENST00000286835 ENST00000399804 ENST00000434667 ENST00000412522 ENST00000438970 ENST00000336418 ENST00000395094 ENST00000252799 ENST00000400113 ENST00000330487 ENST00000480046 ENST00000336023 ENST00000550367 ENST00000549870 ENST00000301071 ENST00000295766 ENST00000550767 ENST00000547939 ENST00000546918 ENST00000552924 ENST00000541364 ENST

Gene Information

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily which is composed of six distinct families. Genes from the alpha beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq Jul 2008]

Gene Symbol:   TUBA1A
Gene Name:   tubulin, alpha 1a
Aliases:   B-ALPHA-1, FLJ25113, LIS3, TUBA3
RefSeq:   NC_000012.11 NG_008966.1 NT_029419.12
Ensembl:   ENSG00000167552
Entrez:   7846
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.040000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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