PrimePCR™ Probe Assay: TMEM25, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TMEM25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMEM25, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041654
Assay Design:   exonic
Chromosome Location:   11:118403746-118403842question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000405493 ENST00000171000 ENST00000319363 ENST00000378896 ENST00000265187 ENST00000378886 ENST00000323040 ENST00000411589 ENST00000442938 ENST00000354284 ENST00000533137 ENST00000526973 ENST00000354064 ENST00000533102 ENST00000313236 ENST00000524725 ENST00000526853 ENST00000359862 ENST00000488353 ENST00000215739 ENST00000415817 ENST00000523944 ENST00000262105 ENST00000520539 ENST00000220959 ENST00000521922 ENST00000433363 ENST

Gene Information

Description Not Available

Gene Symbol:   TMEM25
Gene Name:   transmembrane protein 25
Aliases:   FLJ14399
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000149582
Entrez:   84866
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.910000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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