PrimePCR™ Probe Assay: GGTLC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GGTLC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GGTLC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041653
Assay Design:   exonic
Chromosome Location:   20:23965765-23965866question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000248923 ENST00000400382 ENST00000400380 ENST00000401885 ENST00000404532 ENST00000403838 ENST00000404223 ENST00000400383 ENST00000406383 ENST00000263997 ENST00000404912 ENST00000424787 ENST00000589334 ENST00000206380 ENST00000405188 ENST00000401924 ENST00000424627 ENST00000286890 ENST00000278765 ENST00000335694

Gene Information

This gene encodes a member of the gamma-glutamyl transpeptidase (GGT) family which are important in the metabolism of glutathione. The most ubiquitously expressed human GGT gene GGT1 encodes a single transmembrane polypeptide that is post-translationally processed to form a heavy and a light chain. In contrast the product of this gene only contains homology to the light chain region and lacks a transmembrane domain. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Oct 2008]

Gene Symbol:   GGTLC1
Gene Name:   gamma-glutamyltransferase light chain 1
Aliases:   GGTL6, GGTLA3, GGTLA4, MGC50550, dJ831C21.1, dJ831C21.2
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000149435
Entrez:   92086
Chromosome Mapping:   20p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.470000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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