PrimePCR™ Probe Assay: PCDH11Y, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCDH11Y, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCDH11Y, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041633
Assay Design:   exonic
Chromosome Location:   Y:4925056-4925159question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000333703 ENST00000362095 ENST00000400457 ENST00000215473 ENST00000395337 ENST00000361724 ENST00000373094 ENST00000373097 ENST00000361655 ENST00000406881 ENST00000373088 ENST00000504220 ENST00000298274

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 7 cadherin repeats a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene is located in a major X/Y block of homology and its most closely related cadherin superfamily member is located in this homologous region on the X chromosome. The protein is thought to play a fundamental role in cell-cell recognition essential for the segmental development and function of the central nervous system. Transcripts arising from alternative splicing encode isoforms with N- and C-terminal variation. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH11Y
Gene Name:   protocadherin 11 Y-linked
Aliases:   PCDH-PC, PCDH22, PCDHY
RefSeq:   NC_000024.9 NT_011896.9 NG_011652.1
Ensembl:   ENSG00000099715
Entrez:   83259
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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