PrimePCR™ Probe Assay: LIMS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LIMS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LIMS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041628
Assay Design:   exonic
Chromosome Location:   2:128396653-128396749question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000432532 ENST00000517855 ENST00000395023 ENST00000520958 ENST00000378560 ENST00000395029 ENST00000342854 ENST00000274853 ENST00000443517 ENST00000438815 ENST00000437121 ENST00000455935 ENST00000435572 ENST00000412189 ENST00000426981 ENST00000409286 ENST00000409754 ENST00000324938 ENST00000409455 ENST00000355119 ENST00000409808 ENST00000410011 ENST00000410038 ENST00000545738 ENST00000400554 ENST00000402558 ENST00000379174 ENST00000379166 ENST00000345050 ENST00000525748

Gene Information

This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase) a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains each domain forming two zinc fingers which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   LIMS2
Gene Name:   LIM and senescent cell antigen-like domains 2
Aliases:   FLJ10044, PINCH2
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000072163
Entrez:   55679
Chromosome Mapping:   2q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.690000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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