PrimePCR™ Probe Assay: MCM9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MCM9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MCM9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041593
Assay Design:   exonic
Chromosome Location:   6:119252816-119256073question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000316316 ENST00000316068 ENST00000425154 ENST00000505446

Gene Information

The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds and is a positive regulator of the chromatin licensing and DNA replication factor 1 CDT1. [provided by RefSeq Nov 2010]

Gene Symbol:   MCM9
Gene Name:   minichromosome maintenance complex component 9
Aliases:   C6orf61, FLJ13942, FLJ20170, FLJ56845, MCMDC1, MGC35304, dJ329L24.1, dJ329L24.3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000111877
Entrez:   254394
Chromosome Mapping:   6q22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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