PrimePCR™ Probe Assay: SNRPD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041574
Assay Design:   exonic
Chromosome Location:   19:46190776-46190885question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000342669 ENST00000585392 ENST00000587367 ENST00000588599 ENST00000391932 ENST00000588301 ENST00000590212

Gene Information

The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2009]

Gene Symbol:   SNRPD2
Gene Name:   small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
Aliases:   SMD2, SNRPD1, Sm-D2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000125743
Entrez:   6633
UniGene:   Hs.515472
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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