PrimePCR™ Probe Assay: CLDN24, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041497
Assay Design:   exonic
Chromosome Location:   4:184243040-184243267question
Amplicon Length:   198
Splice Variants Targeted:   ENST00000403733 ENST00000323319 ENST00000514470 ENST00000541814 ENST00000260197

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is 75% identical to the mouse homolog. This gene is upstream of the CLDN22 gene which overlaps the WWC2 gene on the opposite strand in the genome.[provided by RefSeq Aug 2010]

Gene Symbol:   CLDN24
Gene Name:   claudin 24
Aliases:   Not Available
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000185758
Entrez:   100132463
UniGene:   Hs.727475
Chromosome Mapping:   4q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.510000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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