PrimePCR™ Probe Assay: TCEB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TCEB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TCEB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041385
Assay Design:   exonic
Chromosome Location:   16:2826999-2827096question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000408937 ENST00000371172 ENST00000393871 ENST00000419479 ENST00000249016 ENST00000381793 ENST00000381795 ENST00000295971 ENST00000319592 ENST00000285039 ENST00000592779 ENST00000324581 ENST00000378714 ENST00000484800 ENST00000378694 ENST00000291481 ENST00000262306 ENST00000409906 ENST00000409477 ENST00000494946 ENST00000572954 ENST00000419198 ENST00000327773 ENST00000357389 ENST00000540342 ENST00000393590 ENST00000335350 ENST00000373192

Gene Information

This gene encodes the protein elongin B which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2 B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq Aug 2008]

Gene Symbol:   TCEB2
Gene Name:   transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
Aliases:   ELOB, SIII
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103363
Entrez:   6923
Chromosome Mapping:   16p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.910000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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